does the mixed vial require refrigeration?

yes and should be used within 7 days

Once mixed, do you have to use the serum once opened? Or is one bottle for both day/night or can it be used 2x only in the morning for example?

Hi, I get like 4 days use day & night out of one bottle. Keep in a cool place, or in a fridge.

Will this do anything for my scalp for hair loss?

Hello! As this product is a 'facial' product, it is not recommended to use it arbitrarily. We recommend that you use it as indicated in the product name and description. [Premium Skin Care] : Recovering damaged and broken skin by boost-up skin condition. [Hydrating] : Hydrating skin with 3 Moisture Layering System with collagen boost up protein peptide complex. [Freeze & Drying] : Dried microbial reproduction and only remained skin active ingredients which good for skin regenerating and elasticity. [Texture] : Freeze & dried treatment powder type + Transparent liquid ampoule . [Visible Effects] : Recommended to use a vial for a week for intense skin care Thank you for your interest in our products.

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